2 edition of Changes in cytoskeletal organization during myotube formation in vitro. found in the catalog.
Changes in cytoskeletal organization during myotube formation in vitro.
Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1992.
RacGAP50C directs perinuclear -tubulin localization to organize the uniform microtubule array required for Drosophila myotube extension April Development (9) It seems highly likely that the elongation, alignment, and fusion steps that occur during myotube formation also require alterations of the cytoskeleton (Chen et al., ). Finally, netrin-1 and neogenin function in an adhesive, rather than a guidance, role during mammary gland morphogenesis (Srinivasan et al., ), an activity that could Cited by:
The absence of innervation during embryonic development has been shown to result in the degeneration of myotubes, 9 ablation of secondary myotube development, 10 and reduction in myotube size. 11 Furthermore, slow myosin heavy chain expression is reduced in the absence of innervations, 11 which in turn will affect muscle by: The structural organization of microtubule and actin filaments undergo dramatic changes when the cell undergoes what? A tubulin homolog called what was identified that could assemble into ring-like structure at the site of septum formation during cell division? Alpha or beta tubulin. Molecular Biology of the Cell Chapter 13 Part 1
Myoblast fusion is a critical process that contributes to the growth of muscle during development and to the regeneration of myofibers upon injury. Myoblasts fuse with each other as well as with multinucleated myotubes to enlarge the myofiber. Initial studies demonstrated that myoblast fusion requires extracellular calcium and changes in cell membrane topography and cytoskeletal by: Neuromuscular Junction Formation in Tissue-Engineered Skeletal Muscle Augments Contractile Function and Improv es Cytoskeletal Organization. Neil R W Martin 1 Arthritis Research UK Centre for Sport, Exercise and Osteoarthritis, National Centre for Sport and Exercise Medicine (NCSEM), School of Sport, Exercise and Health Sciences, Loughborough.
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Changes in cytoskeletal organization during myotube formation in vitro. (Thesis) Zeytinoglu M. Publisher: University of East Anglia  Metadata Source: The British Library Type: Thesis.
Abstract. No abstract supplied. Menu. Formats. Abstract. EThOS. About. About Europe PMC Author: Zeytinoglu M. Regulation of skeletal myotube formation and alignment by nanotopographically controlled cell-secreted extracellular matrix Alex Jiao,1 Charles T. Moerk,1 Nisa Penland,1 Mikael Perla,1 Jinsung Kim,1 Alec S.
Smith,1,3,4 Charles E. Murry,1,2,3,4,5 Deok-Ho Kim1,3,4 1Department of Bioengineering, University of Washington, Seattle, Washington 2Department of Pathology, University of.
Changes in cytoskeletal organization during myotube formation in vitro. Author: Zeytinoglu, Melih. The cytoskeletal organization of the mouse egg changes during ageing in vivo and in vitro. The earliest change observed is the disappearance of the microfilament-rich area overlying the meiotic spindle.
This is followed by the migration of the spindle towards the centre of the egg. Focusing on the potential role of aligned electrospun fibers in myotube formation, Choi et al.
fabricated μm PCL/collagen meshes with varying degrees of fiber orientation (Choi et al., a). Human skeletal muscle cells—seeded onto fiber meshes—developed longer and more highly aligned myotubes when the fibers were aligned, as. Formation of Cytoskeletal Elements During cells that previously seem to have expressed filaments of the vimentin type [e.g., 14, As has been shown in myotube formation in vitro, desmin filaments appear in myoblast cells containing vimentin filaments [ but it is still not clear whether vimentin disappears late in myogenesis or is Cited by: Thickening of actin fibers is part of the actin cytoskeletal remodelling upon myotube differentiation that also includes stress fiber formation, changes in F-actin organization and the shift in.
Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds This article has been downloaded from IOPscience. Mouse oocytes showed similar morphological alterations and cytoskeletal organization during aging in vivo and in vitro (Longo, ; Webb et al., ) although previous studies by Adenot et al.
() showed that microtubular organization in MII oocytes aged in vivo differed from oocytes aged in by: The ability of a eukaryotic cell to resist deformation, to transport intracellular cargo and to change shape during movement depends on the cytoskeleton.
During myogenesis, myoblasts must exit the cell cycle and subsequently undergo an ordered set of myogenic events, such as migration, cell-to-cell adhesion, and myotube Author: Mi-Ock Baek, Chi Bum Ahn, Hye-Jeong Cho, Ji-Young Choi, Kuk Hui Son, Mee-Sup Yoon.
Myoblast migration. Myoblast migration, best described in studies in vitro, enables formation of groups of elongated fusion-competent cells that then align to achieve cell-cell contact and recognition thus promoting myotube us mechanisms are involved in controlling myoblast migration though nearly all of them are not muscle : Lilya Lehka, Maria Jolanta Rędowicz.
Fetal stage is crucial for skeletal muscle development, when muscle fibers are formed by fusion of mesodermal progenitor cells, myoblasts. During postnatal period, the number of myofibers remains constant; however, the size of each myofiber can increase by fusion with muscle stem cells Cited by: 4.
Duchenne muscular dystrophy (DMD) is a progressive muscle-wasting disease that occurs in childhood and results in early death. Improvements in clinical management (Bushby et al., ; Schram et al., ) have lengthened the life span of DMD patients despite the present lack of an etiologic by: The sequence scheme of the three HELP polypeptides is reported in Fig.
A His-tag sequence is present at the N-terminus (dark gray). HELP and HELPc share the same backbone, composed by the cross-linking (green) and the hydrophobic domains (yellow) of human the newly synthesized HELPc, the backbone of the polypeptide is fused, at the C-terminus, with a 41aa stretch from collagen Cited by: 8.
Moreover, calpain inhibition seems to negatively modulate cell migration by inhibiting formation of new adhesions and destabilizing cytoskeletal linkages.
As previously observed in fibroblasts and in bovine aortic endothelial cells , , the inhibition of calpain in myogenic cells causes a Cited by: The formation of these mixed microtubule polarity arrays re-introduces the ability for kinesin-1 mediated microtubule sliding and thereby neurite outgrowth (Lu et al., ).
In general, severe cytoskeletal rearrangements occur after injury and targeted cytoskeletal rearrangement may be a promising strategy for enhancing axon by: Skeletal muscle is the largest tissue in the body and loss of its function or its regenerative properties results in debilitating musculoskeletal disorders.
Understanding the mechanisms that drive skeletal muscle formation will not only help to unravel the molecular basis of skeletal muscle diseases, but also provide a roadmap for recapitulating skeletal myogenesis in vitro from pluripotent Cited by: Many basic building blocks of the cytoskeleton have been identified and characterized extensively in vitro, and researchers are now using advanced light microscopy to determine, with great spatial and temporal precision, the locations and dynamics of these cytoskeletal proteins during processes such as cell division and by: Table S2: Identified and quantified proteins from differentiating human muscle cells.
Table S3: Proteins differentially expressed with in vitro differentiation. Table S4: Functional annotation enrichment for proteins with similar regulation pattern during myotube formation in vitro. Table S5: Proteins with known muscle diseases by:. The function of Stac3 was further characterized in vitro using the mammalian C2C12 myogenic cell line.
Stac3 mRNA expression increased during the differentiation of the C2C12 myogenic cell line. Knockdown of Stac3 by RNAi inhibited myotube formation, and microarray analysis revealed that transcripts involved in cell cycle, focal adhesion, cytoskeleton, and the pro-myogenic factors Cited by: Skeletal muscle plays an important role in the human body for movement, mastication, and other dynamic actions.
In the presence of severe muscular injuries, current treatment approaches are Cited by: 5.These findings are consistent with published reports that the TWEAK receptor Fn14 is essential for myotube formation in cultures and for the regeneration of adult skeletal muscle .